Genome editing refers to manipulating the nucleotide sequences of the genome with engineered nucleases. Genome editing by engineered nucleases (GEEN) is a valuable genetic engineering approach that uses artificially or modified engineered endonucleases ((EENs) which induce site-specifically double-stranded DNA break (DSB). These EENs contain DNA-binding domain or RNA sequence, which identify its template and nucleases efficiently and precisely cleave the targeted DNA sequences. The double strand breaks (DSBs) of DNA consequently induce the DNA repair mechanisms, results in gene modification at the target sites. These DNA repair mechanism based on error-prone non-homologous end joining breaks (NHEJ) and homology-directed repair (HDR).