DNA barcoding is an efficient molecular approach for identification of nematode species. Among nuclear and cytoplasmic genes, SSU r RNA genes proved to be potential markers for DNA barcoding. Barcoding is highly useful technique for quarantine purpose by increasing the speed of identification to avoid entry of new pathogen in new area. Nematode barcoding is not only useful for species identification, but also for evolution and phylogenetic studies of nematodes. Integration of advance morphological approaches (video capturing) with molecular approach (DNA barcoding) will provide an efficient platform for identification of nematode species which is the need of hour.